Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQHQSLFNKSQWTQLVKHFSAVAVLGLTIAAPAWAIDPTAKELRIGFQKSSINFAIAKQQKLFEQEFPNAKITWNEFPAGPQILEALAVGSLDVGVTGDTPPVYAQAAGKPLYYIAYEAAKPLASAILVPKNSQLKQLKDIKGKRIALQKGSSSHYLLVQ-AVRKAGLK-WSDITPIWLTPADARAAFQKGAVDAWAIWDPYYASAQLEDQARVLVSGKGLSPNY--TFYLAAPNFVKQYPKAVPGLIKQINQADKWV--QSYQVETASAIGQSTGLKPATSDLFIKRRPRPSSAAPLNSKV-----------------IAEQQQIADIFTQQGIIPKPISIKQAVWGAK-------------------------------------------------- 2G29 Chain:A ((28-412)) -------NAPEVTTA-----KLGFIALTDAAPLIIAKEKGFYAKYGMPD--VEVLKQASWGTTRDNLVLG-SASGGIDGAHILTPMPYLITMGTVTDGKPTPMYILARLNVNGQGIQLGNNYKDLKVGTDAAPLKEAFAKVTDPKVAMTFPGGTHDMWIRYWLAAGGMEPGKDFSTIVVPPAQMVANVKVNAMESFCVGEPWPLQTVNQGVGYQALTTGQLWKDHPEKAFGMRADWVDQNPKAAKALLMAVMEAQQWCDQAENKEEMCQILSKREWFKVPFEDIIDRSKGIYNFGNGQETFEDQEIMQKYWVDNASYPYKSHDQWFLTENIRWGYLPASTDTKAIVDKVNREDLWREAAQALEVPADQIPSSPSRGIETFFDGITFDPENPQAYLDSLKI

General information: TITO was launched using: RESULT: Template: 2G29.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1925 -46142 -23.97 -147.89 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -23.97 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_2G29.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G29-query.scw PDB file : Tito_Scwrl_2G29.pdb: