Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQ--GVRGN-GVIR-K----LAAPFK-ILKATFSAMRYMKQLKVDAVAGFGGYVAGPGGLAARLLGIPVLIHEQNAVAGFTN-------------------AQLSRVAKV----VCEAFPNTF-----------PAS----------EKVVTTGNPVRREITDILSPKWRYDEREQAGKPLNILIVGGSLGAK--ALNERLPPALKQLQVPLNIFHQCGQQQVEATQALYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLL-TEMAVKARQHAQPNATQHVVGLIQKM 2IYF Chain:B ((6-395)) -------TPAHIAMFSIAAHGHVNPSLEVIRELVARGHRVTYAIPPVFA--DKVAATGPRPVLYHSTLPGPDADPEAWGSTLLDNVEPFLNDAIQALPQLADAYADDIPDLVLHDITS--YPARVLARRWGVPAVSLSPNLVAWKGYEEEVAEPMWREPRQTERGRAYYARFEAWLKENGITEHPDTFASHPPRSLVLIPKALQPHADRVDEDVYTFVGACQGDRAEE---G----GWQRPAGAEKVVLVSLGSAFTKQPAFYRECVRAFGNL-PGWHLVLQIGRKVTPAEL---GELPDN--VEVHDWV-PQLAILRQADLFVTHAGAGGSQEGLATATPMIAVPQAV----DQFGNADMLQGLGVARKLATEEATADLLRETALALVDDPEVARRLRRIQAEMAQEGGTRRAADLIE--

General information: TITO was launched using: RESULT: Template: 2IYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1672 -67668 -40.47 -202.60 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -40.47 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_2IYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IYF-query.scw PDB file : Tito_Scwrl_2IYF.pdb: