Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDLVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLEAGVTFVIIGTKAVQEPEFVEEACKSFAGHIIVGIDA--MNGMVATDGWANVTDV-KATDLAKRFADAGVSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRNLK--GQLGILGAITGRAIYEGTLNLREAQLLLDENRL 2Y85 Chain:D ((3-238)) LILLPAVDVVEGRAVRL---------EYGS-AVDAALGWQRDGAEWIHLVDLDAAFGRGSNHELLAEVVGKL--DVQVELSGGIRDDESLAAALATGCARVNVGTAALENPQWCARVIGEHGDQVAVGLDVQIIDGEHRLRGRGWETDGGDLWDVLERLDSEGCSRFVVTDITKDGTLGGPNLDLLAGVADRTDAPVIASGGVSSLDDLRAIATLTHRGVEGAIVGKALYARRFTLPQA---------

General information: TITO was launched using: RESULT: Template: 2Y85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1304 -105820 -81.15 -476.66 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -81.15 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.696

(partial model without unconserved sides chains): PDB file : Tito_2Y85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y85-query.scw PDB file : Tito_Scwrl_2Y85.pdb: