Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------MDFNCDLVWMNSMSALLRFTQF----VQKTFALWVIIFAALALWQPEFFVWLKAYIPWIL-------GIIMLGMGMTMTVDDF--------KGVLQSPKAVLIGVVAQFVVMPGLAFILC-------------KLFNLPPEIAVGVILVGCCPGGTASNVITYMAKGNVALSVACTSVSTLLAPVLTPAIFYLLASQWLKIDAASMFISILQ-----VVLLPIVIGLILRTWLKRQVESYIQVMPLVSVIAIVAIVAAIIGGS-------KAAILQSGLLILAVVILHNGLGYLLGFAAARFFKLPYADSKAIAVEVGMQNSGLGVALAAVHFAASPITAV-----PSAIFSLWHNISGPALATYWASKHKQE---------------------- 5F0J Chain:A ((4-461)) GSKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRD--ALAQEYLMECIIQVF-PDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATS-SAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQ

General information: TITO was launched using: RESULT: Template: 5F0J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1346 -142207 -105.65 -444.40 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -105.65 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_5F0J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F0J-query.scw PDB file : Tito_Scwrl_5F0J.pdb: