Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTQRCGWCSDDPLYIAYHDEEWGKPEHDEARLFEMLCLEGQQAGLSWITVLKKRESYRQHFFNHPIADIAAFTDDFLETKLSDAGLIRHLGKLKAIRDNAIAWQALKAQVDDVSKWLWQFVDAAPQNNDVVDYRKAPAQTELSLKLSKTLKKNGFKFVGPTTCYAFMQAVGMVNDHENNCQFKAS 2JG6 Chain:A ((1-183)) --MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGLSWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGYLKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPVTVFSFLEAAGLYDAHLKDCPSKP-

General information: TITO was launched using: RESULT: Template: 2JG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 827 -80927 -97.86 -442.22 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -97.86 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2JG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JG6-query.scw PDB file : Tito_Scwrl_2JG6.pdb: