Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRAISHIDTFQGLILALQNYWAEQGCVVLQPYDMEM---------GAGTFHTATFLRALGPE--T-WNAAYVQPSRRPK----DG-RYGENPNRLQHYYQFQVVLKPNP------DNIQQLYLDSL-KAIGIDTL------------------------THDIRF--VEDNWES-PTLGAWGLGWEVWLN----------GMEVTQFTYFQQV----GG-VECYPVTGEITYGLERLAMYLQGVDSVYDLVWTKGQFGTVTYGDVFHQNEVEQSTYNFEYAPVDKLFELFDFYESEANRLMEAKLPLPAYEQVVKASHTFNLLDARGAISVTERQRYILRVRTLARAIAQSYVQARAELGFPMAEPHLRDEVLAQLKAQAESEAAKAEKN 1YFS Chain:B ((2-248)) -------SLSAHEIRELFLSFFEKKGHTRVKSAPLVPENDPTLLFVNAGMVPFKNVF--LGLEKRPYKRATSCQKCLRVSGKHNDLEQVGYTS----RHHTFFEMLGNFSFGDYFKKEAIEYAWEFVTEVLKLPKEKLYVSVYKDDEEAYRIWNEHIGIPSERIWRLGEEDNFWQMGDVGPCGPSSEIYVDRGEEYEGDERYLEIWNLVFMQYNRDENGVLTPLPHPNIDTGMGLERIASVLQGKNSNFEI--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1YFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 652 -6334 -9.71 -36.83 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -9.71 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1YFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YFS-query.scw PDB file : Tito_Scwrl_1YFS.pdb: