Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKNAYIIDAIRTPFGRYAGGLASVRADDLGAVPIKALMQRNPNVDWEQVDDVIYGCANQAGEDNRNVGRMSALLAGLPYQVPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESMSRAPYVMG--KSDSAFGRSQKIEDTTMGWRFINPKLKELYGVDTMPQTAENVAEQFNVNRADQDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEGLSKLKPVVKADGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPAPAIKKLFKQANLTLDQMDVIELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLGASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIVERVEAL 4C2K Chain:C ((24-414)) --LRGVFVVAAKRTPFGAYGGLLKDFTATDLSEFAAKAALSAG-KVSPETVDSVIMGNVLQSSSDAIYLARHVGLRVGIPKETPALTINRLCGSGFQSIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSL--WVSLTDQHVQL----PMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKT--GKQT-MQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDL--DISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQ-----

General information: TITO was launched using: RESULT: Template: 4C2K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2647 -77272 -29.19 -199.67 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -29.19 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4C2K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C2K-query.scw PDB file : Tito_Scwrl_4C2K.pdb: