Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------MMKLLSFLKNKALGSSIDYKILPRKVKF---DWKDTPIDWIPNQPFASYFINEINNILPAGEFWFCRLYNKVLPRITDEKL--KQDVQAFIRQEAMHANAHTSANKEYLSARNIDIQRNLDIMNYLFTTALADKPFDKEVPQFLQEQWDLFRLGVIATVEHMTCVLGKYALYNKRWEELGADPEMVDLVKWHGSEEIEHRTVAFDLY-----RHLGGGYIPRYYLSLAVIVLVLGLWVDGAAHIMKQDPRFADAAKSRFFAAWVALEWYKISRKDNQVLPNPIWLIAQQIDYLMPWYDPVKEGSTEDAVSYLSQSPAAKRAELQAA-------------------------------------------------------------------------------------------------------------------- 4K70 Chain:A ((2-483)) PGSMEALVRALEEADHAVATVVQSRILEFFMAAGRETPAGVRGLWARALRLACRAYVETGTCEAAVLAENLAGLALWRLRHDWDEGTAPLLELLGVVNGDDTTAALTEAGLRTSAEFGPDAMFRLVSEWCAAFDEALAGARSADDVLAAPRVVPPEQTARALVQPRFATLYDMDFVQDGLRYVAQHTNW---ALPLALAVRQMQNEGLKPLTRALFALTIADEFFHDRQNPTLREQFAEAARAV-----------DEAALVPVG--EVNATP----------RTAVEVRVSAALAHGDAYVRELRPGTVARRLRTDQGVLALL----DPGAQAV----HVAAAADLDHTQVDATGVWEAVQA-----SASPLQVVEALVT----------AGFTRRHCDLLERAVLDRAPRLTDAQRAVGCTAVVGGVVHRLLDDYGPGLDYVRAYTDVADTLEPLYGDVTAALGLPEKGVEHVVRHCMAPRPPTEHVGAARAALLREVAAAERRAGLAHSAAREALNTWLAFRAQSRWGL

General information: TITO was launched using: RESULT: Template: 4K70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1102 -26568 -24.11 -99.51 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -24.11 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_4K70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K70-query.scw PDB file : Tito_Scwrl_4K70.pdb: