Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFLQRSNQHSNNNRLKRMKSHLRVHYFQHIAGEGFGSCYSFLKAHHATITATEFFALPVDLPLEIEALPYIEDVDLLLIMGGTMSVNDEANYPWLKIEKRWIRRYLAAGKPAIGLCLGGQLIANALGAAVSRNRYQELGWSTVQRVPNLPKESFL--LPEKINVMQWHSETF-EIPKGAIHLAENSVCRNQMYQIG-SNVLGFQFHPEMTPKVLNLLLENEQELSIFKGEYVQSLDELHHCDIQKFEQGNQLLNRAIEFVVNQ 1WL8 Chain:A ((1-171)) -----------------------MMIVIMDNGG-QYVHRIWRTLRYLG--VETKII--PNT---TPLEEI-KAMNPKGIIFSGGPSLENT----G---NCEKVLEHYDEFNVPILGICLGHQLIAKFFGGKVGRGEKAEYSLVEIEIIDE-----IFKGLPKRLKVWESHMDEVKELPPKFKILARSETCPIEAMKHEELPIYGVQFHPEVAH----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1WL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 -70415 -79.84 -426.76 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -79.84 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_1WL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WL8-query.scw PDB file : Tito_Scwrl_1WL8.pdb: