Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MTPAIRRTVTF--PEISMERLKSILGAYNGHLKQIEQRLDVKITHRGDVFYIDGEIDAVGRAEALLQRLYEETEASQQISADLLHLLIQSSQTERNFELVGEEMDEHDAPMDVYFQTRKGRINPRGANQKRYVQRILQSDISFGVGPAGTGKTYLAVAAAVDMLERNEIQRILLVRPAVEAGEKLGF-LPGDLTQKIDPYLRPLYDALYEMLGFEKVAKLIERQVIEVAPLAYMRGRTLNHSFVILDEAQNTTPEQMKMFLTRLGFGSRAVITGDITQVDLPRGQQSGLAHALRVLENIKEIHITRFHSRDVVRHQLVQKIVEAYEAWDGEQQRLNAEARAERKARQEALIAENDTAADLQHQDA 5T5I Chain:C ((2-270)) SEIILTPKEQPEVPLEAPNIKPDVFAG---------KSIEEIKNIQIMHGNEVVKLGDFFEVSG----------EPADAPEDI-----KIIIDG---------------------DVYNTKRIGQEMTAG-------EIIVRGNVNMYVGAGMKGGKITVEGNAGSWAGQDMRGGEIEILG--DAGDYVGSSYRGDWRGMSGGTITVHGNADNEIGEYMNGGKIIIKGDVNIMP-----GIHMNNGLIIIEGN----------VVARAGGE----MAGGTIVV---KGMMQEFLAGFKYLGVEKDIEVDG------------EELPGAFYKFEGDHAIKGAKGIVYAAVGCNGHIAP------------

General information: TITO was launched using: RESULT: Template: 5T5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1719 79256 46.11 302.50 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 46.11 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_5T5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T5I-query.scw PDB file : Tito_Scwrl_5T5I.pdb: