Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPQYKAPLRDMQFVLHELLNAEEHYAKLPDFQGNVSRELVDQYLEAAANFCENELSPLNQVGDREGCTWNDGVVTTPTGFKEAYQKYIELGFPSLSAEEQYGGQGLPNSLGIAISEMVGSSNWAWGMYPGLSHGAVRTLEHHGSEEQKNTYLPNLVSGV-------------WTGTMCLTESHAGSDLGIIRTKAEPNADGSYAISGEKIFISAGEHDMAENIIHIVLARLPGAPKGTKGISLFIVPKFNVNADGTLGERNAVRCGSIEHKMGIHGNATCVINFDQAKGYLIGPENRGLNCMFTFMNTARIGTAVQGLSASESSFQGALTYAKERLAMRSLSGPKAPEKEADPIIVHPAVRNMLLTQKAFAEGGRALVYLLAQYADIVEKGETEEERKFADNILSLLTPIAKAFLTETGSESAKHGVQVFGGHGFISEHGMEQIVRDTRIACLYEGTTEIQALDLLGRKVLQTQGAMLRDFTKIVHKFVEANKDNAAMKEFVEPLAALNKEWGDLTMQIGMRAMQNPDEVGAAAVDYLYFSGYVTLAYLWARMALVAQEKLAAGTTDVDFYNAKVTTARFYFKKILPRVRSHVDVIAGGLDPLMALDAEHFAF 5EZ3 Chain:D ((47-534)) -TNAYLGDPLLMQIAARFPK-E-LHTELEQAGRFV----LSAEAQDLARLANT-ELPKLRTHDRQGRRI--DLVEYHPAYHALMRRSVAQGLHSSIWEDNPLESGRRHQARAARFYLTAQLEAGHLCPLTMTSAS--LAALMASPEVYKQWSPAVLSRKYDFSQKPAFRKQGVTLGMGMTEKQGGTDVRANATRAEPAIGGAWRLTGHKWFMSAP-M--SD--AFLTLAQTKE------GLSCFLLPRLGEK-----GESNGFFFQRLKDKLGNRSNASSEVEFDGALGQMIGSPGEGVKTIMDMVTLTRLDCAVASAGLMRSGLAEAVHHSRHRHVFG------------KPLVEQPLMQRVLADMALDVAGATALSMRLARAFDMAASDR------AEAAFARSMTPVVKYWVCKIAPALLYEAMECLGGNGYIEDGNLARAYREAPVNAIWEGSGNVMALDVA-RVLSRAPAL-FDGVLDWISGQLGP-----R-------GQGTIDVLRAALQ--------LTETDQGVARLLTEQLAFAAAAAELRQLGAD---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EZ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2586 -49241 -19.04 -103.67 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -19.04 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_5EZ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZ3-query.scw PDB file : Tito_Scwrl_5EZ3.pdb: