Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPVSGAKVGGKIELDFASSDNDKSENVRIRHAYLTYN-----NWLFGQTTSNFLS-SHA-PEMIDF-STNI------GGGTTRIPQVRYNYKLAPATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYAD---GNGNASARAFVENYKSG----------------------------------------AGDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKIL-----------------P------NLRSTLAYGAQFADDGTDYAK--NNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEG--KSYKDNRVGLMAKYSF 2O4V Chain:B ((17-411)) ----------------------------------------------------------------------------------------GLEVATTDKEFSFKLGGRLQADYGRFDGYYTN----------NGNTADAAYFRRAYLEFGGTA---YRDWKYQINYDLSRNVG-NDSAGYFDEASVTYTGFNPVNLKFGRFYTDFGLEKATSSKWVTALERNLTYDIADWVNDNVGTGIQASSVVGGMAFLSGSVFSENNN---DTDGDSVKRYNLRGVFAPLHEPGNVVHLGLQYAYRDLEDSAVDTRIRPRMGMRGVSTNGGNDAGSNGNRGLFGGSSAVEGLWKDDSVWGLE--GAWALG-AFSAQAEYLRRTVKAER-------------DREDLKASGYYAQLAYTLTGEPRLYKLDGAKFDTIKPENKEIGAWELFYRYDSIKVEDDNIVVDSATREVGDAKGKTHTLGVNWYANEAVKVSANYVKAKTDKISNANGDDSGDGLVMRLQYVF

General information: TITO was launched using: RESULT: Template: 2O4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1730 82410 47.64 264.98 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 47.64 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.019

(partial model without unconserved sides chains): PDB file : Tito_2O4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O4V-query.scw PDB file : Tito_Scwrl_2O4V.pdb: