Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLKVCTLLPCSLVIAACSVNSSSTPPTL-SSSTPQVQKSSIEI-SCQDLQN----PAYRQAVLNAINQIRQDSRQCGQQYFSAAKPLSWNNNLYQGANAHS-KDMANNNFLGHVGSTGLDLRARLKKYHMLSKANGENVASGQK---TLNEVLAKWIASPLHCS------NIMNPRYTEYAIACASDQSAKQRS-YWTQQFAGF-- 5F3Q Chain:A ((2-193)) ----------------------SSTTLHNAMQYTAFDVLSSILNLMKADPLYDLLQDQEYEKNEFYGDSYLEERASSLVLKFLRKYEQIPFEM--YSGLRIHTVKNQTLGEIFDLLHLGDTKTFEKKKKGDLVESLIGGCVLLSQRENATLFLLFAHALIDYIFYHSSYIYFNANPPKLVKEEIITDIQNWFKDKLFYYRSSLEKYQT

General information: TITO was launched using: RESULT: Template: 5F3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 644 31902 49.54 193.35 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 49.54 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_5F3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F3Q-query.scw PDB file : Tito_Scwrl_5F3Q.pdb: