Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MRDRIMQITIRGHHLAITPAIEENIKAKFNQLTKHLDQVNSMQIKLTKDHQIDKRSHKGSSNHVAEAIVRLPGIELFAQATADDMYTSIKK-LTEKLKKQLLKYRKMQCTYSQVAVSI 5IHF Chain:A ((6-115)) AVPNPPLPAQDPIVQ------HLKLTNDQITRIKKLHQQLETDVSQISM------GALIEVIKSGKWDDAAVKQQLAAFSNIEQQARYYRVKYYFDLSKVLTPEQRQQVQQDLAQAL---------

General information: TITO was launched using: RESULT: Template: 5IHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 260 11889 45.73 123.84 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 45.73 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_5IHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IHF-query.scw PDB file : Tito_Scwrl_5IHF.pdb: