Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATAVNCNICFIGGGNMAQALIGGLISRG-LPPTRITV---SDPVEQIRQLLQEKEVHVTQDNTAAIQNADVVVLAVKPQVLANVLRPLKGLLS-DKLVISIIAGAEIKTISNLIDSERIVRVMPNTPALVQTGAHGIYATDVVGASDRELTSQILAATGLTIWVNSEAQIDAVTAVSGSGPAYFFYLMESMIRAGKNLGLDEKVATALTLQTALGAAQMAITSSNTPSELRKNVTSPNGTTQAALEVFDRAQISQNIQSALAVAQKRSQELAQELSDSAK 5BSG Chain:J ((11-277)) ---ADSYTLGFIGAGKMAESIAKGAVRSGVLSPSRIKTAIHSNPAR--RTAFESIGITVLSSNDDVVRDSNVVVFSVKPQLLKDVVLKLKPLLTKDKLLVSVAAGIKMKDLQEWAGHERFIRVMPNTAATVGEAASVMSLGGAATEEDANLISQLFGSIG-KIWKADDKYFDAITGLSGSGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATQSGKHPGQLKDDVTSPGGTTIAGVHELEKAGFRGILMNAVVAAAKRSQELS--------

General information: TITO was launched using: RESULT: Template: 5BSG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 1103 -11737 -10.64 -44.80 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain J : 0.81 3D Compatibility (PKB) : -10.64 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_5BSG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BSG-query.scw PDB file : Tito_Scwrl_5BSG.pdb: