Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRERMEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGEKIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQNGAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISLKLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQGGYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK 4C03 Chain:B ((87-122)) ----------------------------------------------------------------------------------ALRGKTVLDVGAGTGILSIFCAQAGARRVYAVE-----------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4C03.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 99 -6308 -63.72 -191.15 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -63.72 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.647

(partial model without unconserved sides chains): PDB file : Tito_4C03.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C03-query.scw PDB file : Tito_Scwrl_4C03.pdb: