Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEELTSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLHVVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSITAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVADSSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPEREGKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQMFIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLYWTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGISGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLSVFLTLSCISLALFVLGS-IGYRRRDLLEG- 4BWZ Chain:A ((3-386)) --------------------------------GAEHLLEIFYLLLAAQVCAFIFKRLNQPVVIGEV--LAGVLVGPALLGLVHEGEILEFLAELGAVFLLFMVGLETRLKDIL-------------------------------AVGKEAFLVAVLGVALPFLGGYLYGLEIG----FETLPALFLGTALV--ATSVGITARVLQELGVLSR------------------------------------PYSRIILGAAVIDDVLGLIVLACVNGVAETGQVEVGAITRLIVLSVVFVGLAVFLSTLIARLPLERLPVGSPLGFALALGVGMAALAASIGLA---------PIVGAFLGGMLLSEVREKY---------------RLEEPIFAIESFLAPIFFAMVGV-----RLELSALAS---------PVVLVAGTVVTVIAILGKVLGGFLGALTQ--GVRSALTVGCGMAPRGEVGLIVAALGLKAGAVN-------EEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

General information: TITO was launched using: RESULT: Template: 4BWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2150 -363754 -169.19 -952.23 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -169.19 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_4BWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BWZ-query.scw PDB file : Tito_Scwrl_4BWZ.pdb: