Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------GCQAELLNINQAVVGTGCVQMNYYANIYDSTR-------------RAGYTVNANSNCG---------------LSFGNIQVIP------DGYSLRQAGYC------------------------------------------------------------------------------------------------------------ 1ELV Chain:A ((70-328)) CGVPREPFIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQKMLTPEHVFIHPGWKLLAVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENS

General information: TITO was launched using: RESULT: Template: 1ELV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 184 2889 15.70 43.77 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 15.70 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.146

(partial model without unconserved sides chains): PDB file : Tito_1ELV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ELV-query.scw PDB file : Tito_Scwrl_1ELV.pdb: