Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAYIPKNYIVKANGNSVITGTEFLSAISSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQASSLNKQLVKLRAKEAIFQKFEQSLNEKYNRMSNSGEEQEYYGKVEYYLSQLNSENYNNGTQYSKIQDEYTKLNKITAERNQLDADLQTLQNELIQLQQQGDSPSLSDTTSADDKAKLETKILEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSELGTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLVPLKQGMSIQQGQT--IAEVSGKEKGYYVEAFVLASDISRVSK----GA---------KVDVAITGVNSQKYGTLKGQVRQIDSGTISQETKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ 2V4D Chain:G ((22-263)) -------------------------------------------AQTVTLNTELPGRTNAFRIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQAQRY----------------------------------------------------------------------------KLLVADQAVS----------------------------KQQ-------------------------------------------YADANAAYLQSKAAVEQARINLRYTKVLSPISGRIGRS-AVTEGALVTNGQANAMATVQQL-DPIYVDVTQPSTALLRLRRELASGQLERAGDNAAKVSLKLEDGS---QYPLEGRLEFSEVSVDEG------TGSVTIRAVFPNP-------NNELLPGMFVHAQLQEGV---------------

General information: TITO was launched using: RESULT: Template: 2V4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 871 -7148 -8.21 -31.49 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain G : 0.60 3D Compatibility (PKB) : -8.21 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_2V4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4D-query.scw PDB file : Tito_Scwrl_2V4D.pdb: