Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLAILLITISETLP---QVLQIFL-----GVLADFQHHRVLKYTVISFAKFLLYSIVSL----SLSGQSFSLLLVAF-ICLINLLSDTLSYFSGAMLTPIFI--RIIGQDHLAEAIGFKQSTVSLVKTISNILGGVL---LGILSIQFISLLNALTFLI--AFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLL----QSSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNL---QTGQSLA-LLSTFELSALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGVLSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLLFLLVAFVMLLNIRESI 5TF6 Chain:A ((1-367)) MLT---RNRELTTVLVKNLPKSYNQNKVYKYFKHC--------GPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHKVVGQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLSTELLDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCAFMVFENKDSAERALQMNRSLL-------GNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHIN----EKDIRKILLVSDF----NGAIIIFR--DSKFAAKMLMILNGSQFQG-----------------KVIRS-------------GT-----------INDMKRYYNNQQN-

General information: TITO was launched using: RESULT: Template: 5TF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1486 -106577 -71.72 -314.38 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -71.72 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.233

(partial model without unconserved sides chains): PDB file : Tito_5TF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TF6-query.scw PDB file : Tito_Scwrl_5TF6.pdb: