Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPKEHYRNLL-----EMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL-----VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA 4EGU Chain:B ((5-115)) --DCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKKHYDSLIDIPDKEMDIV--SHIHVVINKIAKE--KGFDQTGFRVINNCGSDGGQEVKHLHYHILAGKKLPNYE-----------------------------

General information: TITO was launched using: RESULT: Template: 4EGU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 402 -42617 -106.01 -421.95 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -106.01 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_4EGU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGU-query.scw PDB file : Tito_Scwrl_4EGU.pdb: