Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMERNINMKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKTSAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAGDDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAFIDKEGKLVKTHPGFMEKDAILQTLKELS 2H19 Chain:B ((4-129)) ----------------------------------------------------------SDAPNFVLEDTNGKRIELSDLKGKGVFLNFWGTWCEPAKKEFPYMANQYKHFKSQGVEIVAVNVG----ESKIAVHNFMKSYGV-NFPVVLDTDRQVLDAYDVSPLPTTFLINPEGKVVKVVTGTMTESMI--------

General information: TITO was launched using: RESULT: Template: 2H19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 574 -82530 -143.78 -655.00 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -143.78 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.658

(partial model without unconserved sides chains): PDB file : Tito_2H19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H19-query.scw PDB file : Tito_Scwrl_2H19.pdb: