Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYLREQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAVLRYYQLLGDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYIVVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVSMIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFLLFRGFKKIKWSEVQSKSRCKAVFCYNEGKNVRN 5TSZ Chain:A ((13-142)) ----SSLEGGSEFSERIGNSLSSFLSE-SASLEVIGNELADNIANEIVSSLQKDSASFLQSGFDVKTQLKATAKKVLVEALKAALEPTEKIVASTIKPPRVSEDAYFLLGPVVKTLFNKVEDVLHKPIPDTIWEY------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TSZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 327 -5673 -17.35 -43.63 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -17.35 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.137

(partial model without unconserved sides chains): PDB file : Tito_5TSZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TSZ-query.scw PDB file : Tito_Scwrl_5TSZ.pdb: