Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLV-PTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFF--GITADNE---FVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNL------DRFVEAGACGTAAVISPIGGIQH-GDDFHVFYSETEVGP-VTRKLYNELTGIQFGDIEAPEGWIVKVD 2COJ Chain:B ((68-378)) -----------------------------WEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVP-YSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYF----FNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDH-QITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLTTALEGNRVREMFGSGTACVVCPVSDILYKGETIHI--PTMENGPKLASRILSKLTDIQYGREE----------

General information: TITO was launched using: RESULT: Template: 2COJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1474 -74169 -50.32 -253.13 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -50.32 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_2COJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2COJ-query.scw PDB file : Tito_Scwrl_2COJ.pdb: