Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQ--LLKETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYY---QDEADLPAIYTVVQEQERMLGR----KIAVEVEQLR-----HYILAEDYHQDYLRKNPSGYCHIDVTDADKPLIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPLFFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPRELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK 4D7L Chain:A ((66-226)) ---IYIGIGCYWGAEKLFWETPGVVYTSVGFAGGITPNPTYRETCTGRTNHTEIVEVVYDPTQVTFDELVVKAMEAHDPTQGYRQGNDTGTQYRSAIYTAGPNAEQQAQRAREIVEHYAPKLAAAGLGRITTEILPLASTPAGEYYMAEDEHQQYLHKNPLGYC------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4D7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 733 -17204 -23.47 -117.03 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -23.47 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_4D7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D7L-query.scw PDB file : Tito_Scwrl_4D7L.pdb: