Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAYGHGAVAVAKAIQDD-VDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFTLTVAGLEWIQALLDKEVDLTGL-TVHLKIDSGMGRIGFREASEVEQA-QDLLQQHGVCVEGIFTHFATADEESDDYFNAQLERFKTILASMKE----VPELVHASNSATTLWHVETIFNAVRMGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIATVPIGYADGWTRDM-QNFSVLVDGQACPIVGRVSMDQITIRLPKL--YPLGTKVTLIGSNGDKEITATQVATYRVTINYEVVCLLSDRIPREYY 4Y2W Chain:B ((7-375)) ------RPTRVEVYLDAITHNFREIKKIVGKNVKIMAVIKGDAYGHGASYVAKFLEKEGVDYFGVATTEEALELREKGIKTPILIFGYTPPTQLRQIVKHDLTQTVYDIKYAKELEKESLKQNKRAKVHIKIDTGLGRIGYIDFDLAQKEILEMANMRGLILEGIYSHFAAASEDDRDYCKEQFDKFMNLISSLEKKRLKIP-LKHIANAAAILNLNYSHLDMVRPGIILFGAYPSKRV-ERKVELRETLRFTTRVVHLKDVPAGFFIGYGKSFVTKRKSVIATIPVGYADGLDRRLSNNYKLLLKGKYVPIVGRVCMDQCMIDVTDVEGVEIGDEVVIIGTQNNETVSVESMADKIETIPQEVFSRISRRVPRVYF

General information: TITO was launched using: RESULT: Template: 4Y2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1997 -45516 -22.79 -126.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -22.79 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_4Y2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y2W-query.scw PDB file : Tito_Scwrl_4Y2W.pdb: