Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSA-KGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLL-ATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 5DFF Chain:B ((20-276)) LKICSWNVDGL---------RAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAE-----LQEL-PGLSHQYWSAPS-----YSGVGLL-SRQCPLKVSY-GIGD-E-----GRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYM-MNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

General information: TITO was launched using: RESULT: Template: 5DFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1402 -27000 -19.26 -109.75 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -19.26 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_5DFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFF-query.scw PDB file : Tito_Scwrl_5DFF.pdb: