Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MELHFNLELVETYKSNS-QKARILTED--WVYRQSYCPNCGNNPLNHFENNRPVADFYCNHCSEEFEL-KSKKGNFSSTINDGAYATM--MKRVQADN---NPNFF---------FLTYTKNFEVNNFLVLPKQF-VTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDGQVRD---PEKVTKE-------------------------------FKQGLFLRKSSLS---------------------SRGWTIEILNCIDKIEGSEF-------TLEDMYRFESDL--KNIFVKNN---HIKEKIRQQLQILRDKEIIEFKGRGKYRKL---- 4OCV Chain:A ((24-378)) TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1046 35591 34.03 140.12 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 34.03 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.136

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OCV-query.scw PDB file : Tito_Scwrl_4OCV.pdb: