Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------MPQVKLKEGEPVDVAIRRFKRSCEKAGVLADVRKREFYEKPTQERKRK-----KAAAVKRYQKKLARESVRTTRLY--------------------------------------------------------------------------------------------------------------------------------------------------------------- 5DWB Chain:A ((1-278)) MRLDLDFGRGLVAHVMLDNVSEEQYQQISDYFVPLVNKPKLKSRDAIGQAFVMATEVCPDANPSDLWHHVLYRIYIREKIGTD---------PSQSWVRTSGEAFEVALVERYNPVLARHGIRLTALFKGQKGLALTRMGVADRVGSRKVDVMIEKQGGGRSPDAEGFGVVGGIHAKVSLAERVSDDIPASRIMMGEGLLSVLSTLDVKSFPPPHGDLVNRGELGTPDRPSDKRNYIEGHGDFSACFSYNLRTSPSNATTPSGRHIYVSGFSGQDDEFTDYLVAQLA

General information: TITO was launched using: RESULT: Template: 5DWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 139 1366 9.83 22.03 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 9.83 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_5DWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DWB-query.scw PDB file : Tito_Scwrl_5DWB.pdb: