Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKKPVPKIYRTTN---WSSYNQALIKRGNI---SIWFDP---KTQWYAQ-PQGKHGRNQTYSDAAIQCCLMIKSLFRLSLRMVTGFAQSLIKLCGLNWTAPDYSTLCRRQKRIDIAISYQQ-SRDGLHLLVDSTGLKFLGEGEWKRKKHQPAYRRQWRKLHIGIDAKTLQIRAVQLTTNNVSDSQVLGDLLDQIPLDERIDSVYTEGAYDTKHCRQVILD-RDAHAVIPPRKNAKPWKDKKLRSLERNELLKTVKRLGRSLWKKWSGYHRRSLVETKMHCIK 2PNN Chain:A ((11-258)) LYDRRSIFDAVAQSNCQELESLLPFLQRSKKRLTDSEFKDPETGKTCLLKAMLNLHNGQN-----------DTIALLLDVARK-----TDSLKQFVNASYTDSYY----KGQTALHIAIERRNMTLVTLLVENGADVQAAANGDFFKKTKGRPGFY--FGELPLSLAACTNQLAIVKFLLQN---SWQPADISARDSVGNTVLHALVEVADNTVDNTKFVTSMYNEILILGAKLHPTL----KLEEITNRKGLTPLALAASSGKIGVLAYILQREIH-------

General information: TITO was launched using: RESULT: Template: 2PNN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1146 80040 69.84 342.05 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 69.84 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_2PNN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PNN-query.scw PDB file : Tito_Scwrl_2PNN.pdb: