Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRIETEASAHKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWGVPTRNQAKYNFGTYVKVFMPEMLKQVRIHS--GQQQLSLHGWSLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFKLTAPIGNLKTYWQLLKNLDNR--EFIVDHATSSSFI--DNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDIDCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPSGHMGVVSGSQAPTSVWPEMSNWLAMRSD 4BZW Chain:A ((1-275)) MQVEQRTLNTAAHPFQITAYWLDQ-----------------------------------------------ISDFETAVDYPIMIICP-GGGFTYHSGREEAPIATRMMAAGMHTVVLNYQLIVGDQSVYPWALQQLGATIDWITTQASAHHVDCQRIILAGFSAGGHVVATYNGVATQPELRTRYHLDHYQGQHAAIILG--YPVIDLTAGFPTT-------------------------SAARNQITTDARLWAAQRLVTPASKPAFVWQTATDESVPPINSLKYVQAMLQHQV-------ATAYHLFGSGIHGLALANHVTQ-----KPGKDKYLNDQAAIWPQLALRWLQEQGLLAGN---------------------------

General information: TITO was launched using: RESULT: Template: 4BZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1591 -86385 -54.30 -321.13 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -54.30 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.337

(partial model without unconserved sides chains): PDB file : Tito_4BZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BZW-query.scw PDB file : Tito_Scwrl_4BZW.pdb: