Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCEFISEYSEFQYDFISLAQQRFVS----VPEIDLRTM---------------IQNFGDWYFANESSSL-NTPSIWLVK-WFSWVQN--------------NEKQVAANRKKQEQITSTCQ-KPQESGYFANL-----FEEQSESQIVDVT-PAKKFPMIEEVGHA- 4DMI Chain:A ((4-176)) QFRIDSESIRDKLNTLLPSQSRLSGSTTIIPVVDLTETAEGGAQREDLQKAFTLINTIDFDVENTTTTIANTPGFYKVVGNLSSRDEASGAIAVIEVTDGITTKILANNRIVSPDGTTAVQSVPVPFDLMVKLVAGDTLQARSNNAEVRVQGIARQIADVSGNLINP

General information: TITO was launched using: RESULT: Template: 4DMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 221 5491 24.85 44.28 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 24.85 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.117

(partial model without unconserved sides chains): PDB file : Tito_4DMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DMI-query.scw PDB file : Tito_Scwrl_4DMI.pdb: