Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAANVIVYSTSVCPYCVRAKQLLERKGVAYKEVNLS--VEAPEVRAELMQRTNHRTVPQIFINDQFIGGFDQLYALEREGKLDELLA 2E7P Chain:A ((19-103)) -SAPVVVFSKTYCGYCNRVKQLLTQVGASYKVVELDELSDGSQLQSALAHWTGRGTVPNVFIGGKQIGGCDTVVEKHQRNELLPLL-

General information: TITO was launched using: RESULT: Template: 2E7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 -20845 -63.55 -251.14 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -63.55 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.914

(partial model without unconserved sides chains): PDB file : Tito_2E7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E7P-query.scw PDB file : Tito_Scwrl_2E7P.pdb: