Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKGSFMPASTSQYYTRTAQILHWVMAIIFITAWVIGFYSGNFLSYDVDGSFKGDVITLHKNIATTIIFLVVIRLFWRYTHPAPQLPDTMSPTMKTLAHI--------------------GHLLLYVILVALPVTGC-LFSWSAGHPAPVLYLFEIPRLVQDNPELLAIVKPLHIYISW----FAGFLIVGHV--LAALKHHFVDKDNVLNSMTKQPK---- 1KQF Chain:C ((2-217)) -------SKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLTQTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHKVADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGIILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI

General information: TITO was launched using: RESULT: Template: 1KQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 609 -95107 -156.17 -514.09 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : -156.17 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.133

(partial model without unconserved sides chains): PDB file : Tito_1KQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KQF-query.scw PDB file : Tito_Scwrl_1KQF.pdb: