Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEVEQQPTSAPSSPKKRRILRSFLLTILIILLLLVASIIIMMSTDRGSRFLLDRVLQAQQVIKYEYEGGNLLRGIILKNIIAQLKEVDVTLDRADVRLGWRSLILEKEVHLSNADIRNLVIINKAPPSDKPFEFKPIKLPFVLRVDVGDVDHLEIKNSG---SVVNFHDVHLNDALWSETKLKFENSSMDMGYLSVHNATGN-MDFSGKYPLNATADLRIPSLKSLNIQNIKVAAWGSLDTLQAGVATTTPDLLTGWVVLHPVRHEVPMQGALLLKNYHLPLLVEQKLFAKNGVIKFQGDIKQLNLALETDLKGENLPEGQYNALMNTDLVHQLNITDFNGQVMKGAVNLKGLVNWKDHVTWDIKGRLDHVNPKDKAIPQVVQDFLPPSLDAAVSSTGSLEKGTEVFANVDFDRYESWKLKLNQAPEKNKKPQPMFMNVAWANIDRAMPYIGWLSSGSGQVDLTLRDGQQDIKVSTKVYQHEQTLLPAGQYLATLNVK----DNILNVPSFSFAAQKGSLTGQAKALLPSEKRQLAWNALLNAKDFNPQSIHTAAPVNLLNGSIKASGFAKPSQQIIQLEKIDLKGRLAQAGQEMVALAGKSTAALLFNDVKAGGGFKGFAVNYDGSLKALNQANGLLKFSIAGTPEFIRISQLQHDGVAGKIYATGALNLKDRIAWDINSSLVRFKPQYFASSVKGEISGNVKTQGIWSDKLKR--IDIQQLNLA--GYLNNKPVRGKGNLSILMDSNQKGFLPQQFEANNLFLVYAQNQLQATGNAQNLKIKLNAPALYELYPGLSGRAYGDLSVQSQPRLKATANIAVDNFAFNTLVSIKKLRIQGELPTSETTPTQLTAKLDNLRSGSRQIQSAEVNLTGTRKAHLLKVQGNNNV-SKFYVQLAGGFNAKNDWLGQIQKGSFDSRRIRLAQNQNAPVVFSSARSELYVGQHCWQSTNSQLCLDQPVRVSKAQGNISFVTQNLDLGDFAAFMPEGLAMTGQLNGYAKASWVNGGHPKLDARLVTRKGELGLAAEDPQDPPTTLAYDELSVIAKSISEGLLFRVDVKTPDIGTGYANVIINPYQSSMPMHGEVAFNDVQLKVLKPFIQDVRSMSGTLALAGKVNGTLTQPQFTGEMRLKNGAISMISLPVNLTNVQVYSSIRQDMATIDGAFNSGQGVGLLKGSFDWKNAPRLQLNLKGDNLLVRQAPLITAIANPNLTLDMYPFDKRLSLKGSVDVPRARISMPETTAPVVNTSSDVRIVRQGQDPLAILRAAKPWDIRADISVNIGNQVIFQGFNSNIPLVGRLNLTQRGYETAMRAMGAIGVSQKVKIEAYGQSLDLNRAIARFNGPLANPTLDIDANKNVQGSMVGVRVTGTASSPNIQVYNDAGLSEQEALNALVTGRINEGASGLSNAEGFKSDVNNTIAAAGISMGLGGTRALTNQIGRTFGLSGLALDAQGTGDDTQVSLTGYITPDLFIRYGVGVFTPVNKLTLRYQMNRRLYLEASQSLERAIDLFYNWRF 4W8J Chain:A ((33-1178)) -------YTPIDISLSLTQFLLSEFVPGAGFVLGLVDIIWGIFGPSQWDAFLV----QIEQLINQRIE----------------------EFARNQAISRLEGLSNLYQIYAESFREWEADPTNPALREEMRIQFNDMNSALTTAIPLLAVQNYQVPLLSVYVQAANLHLSVLRDVSVFGQRWGFDAATINSRYNDLTRLIGNYTDYAVRWYNTGLERVWGPD----------SRDWVRYNQFRRELTLTVLDIVALFPNYDSRRYPIRTVSQLTREIYTNPVLE-----------NFDGSFRGSAQGIERSIRSPHLMDILNSITIYTDAHR--GYYYWSGHQIMASP----VGFSGPEFTFPLYGTMGNAAPQQRIVAQ-----LGQGVYRTLSSTLYRRPFNQQLSVLD-------GTEFAYGTSSN------------------LPSAVYRKSGT-------VDSLDEIPPQNNNVPPRQGF---SHRLSHVSMFRSGFVSIIRAPMFSWIHR------------------------------------SAEVNNII-----------ASDSITQIP--AVKGNFLFNG-----------------SVISGPGFTG------GDLVRLNSSN-------IQNRGYIEVP----------------------IHFPSTSTRYRVRVRYA-SVTPIHLNVNWGNSSIFSNTVPATATSLDNLQSSDFGYFESANAFTGNIVGVRNFSGTAGVIIDRFEFIPV-----TATLEAEYNLERAQKAVNA-----LFT--STNQLGLKTNVTDYHIDQVSNLVTY------LSDEFSLDEKRELSEKVKHAKRLSDERNLLQDSNFKDINRERGWGGSTG---ITIQGGDDVFKENYVTLSGTFD--------------ESYPTYLYQKIDESKLKAFTRYQLR----------------------------GYIEDSQDLEIYLI------------------------------RYNAKHETVNVPGTGSSAHHSHHFSLDIDVGSTDLNEDLGVWVIFKIKT------------------QDGHARLGNLEFLEEKPLVG--EALARVKRAEKKWRDKREKLEWETNIVYKEAKESVDALFVN----SQYDQLQADT-NIAMIHAADKRVHSIREAYL----PELSV-----------IPGVNAAIFEELEGRIFTAFSLYDAR--------------------------NVIKNGD----FNNGLSSWNVKGHVDVEEQNN-------------QRSVLVVPEWEAE--------VSQEVRVSP--------------------------------GRGYILRVTAYKEG-----YGEGSVTIHEIENNTDELKFSNP-------------------------------------------------------------VGYVTKELEY------FPETDKVWIEIGETEGTFIVDSVELLLMEE-------

General information: TITO was launched using: RESULT: Template: 4W8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 5286 95580 18.08 95.20 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 18.08 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.088

(partial model without unconserved sides chains): PDB file : Tito_4W8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W8J-query.scw PDB file : Tito_Scwrl_4W8J.pdb: