Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------MNSFMQQSLKMKKILMMSMCGALLLTGCNKTSQQDQ-----TVSKEQKSTAQKTTEQRDSSCLQIMVAMHTINQNSKIEDLNQINEKLKTCVPSLKSEEQLKLIEASTA-----MYQRFLKQDYTDKTARAFEA---FGYAVLEQKQDLKKVIQSQK---------KLFAQLSPRDQYLLQHEGQAYIELLYQGEGMFTYRRQPNYLI-----DVFSKALPADQKEFLSRMAKDNQDIFYNDGALAVSWK--ELTERALFWEKFIQKYPK---------SYFINDAKLL---FNEYRYFIFFGLDNTPVSNEYAPNTWF-DPDALQQIRFLSTQSQSS--LAKP--------AQKFLKFIATPVDERNKQFKIDLTDENGQKKS-----TYQIVH-----EQLEQLLKFDSPWNTEIYRDCH-------------IDAVCIDTN------------------------------------------- 3L2P Chain:A ((1-579)) HMRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSL-LTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVELSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSK

General information: TITO was launched using: RESULT: Template: 3L2P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1653 87454 52.91 237.65 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 52.91 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_3L2P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L2P-query.scw PDB file : Tito_Scwrl_3L2P.pdb: