Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMALKVSIASKSAHLTPEQIEEFGRRVEQIRQDVMQSLGEQDAKYIYKVRNFVRYTEIASRGMLMFGGWIPPVWLLGTGLLGISKIVENMELGHNVMHGQFDW----LN-EPSLNGNTYDWDTIASGDDWRETHNYVHHTYTNIVGKDHDIGYGILRVSDQQKWE-PRHLF------NIPLALQLMFF------FEWYVGV-------QNLHLEDAL-VYKT----KSW-KQVWEDAAKVRKKATRQVLKD--YVFFPVISGPMFLPVFAGNVV---ANIIRNLWSSAVIFNGHFTEDAETFEPDNTDTETKAEWYLRQIRGSSNFSGT-E--------WLHFMSGNLSHQIEHHLFPDMPAN------RYKEVAPKIKALCAEY----GINYNEA-N-FMRQFWSVWVRLAKCSLPNHTTAKVMQTLEKLKAKFKFA 4DN7 Chain:A ((24-428)) ---QTEQVSLKKRAESAAEKKAAFGEDFELEKYEEGSKVSKP-------IEDLQSLDEESKKTLLQVG--VIPS---EEGRSGSFLVLDNAVSHSTLKDKNVELMSTHKAMEKYEWLKDYSWKLVQVDAD----------KYTAKTYLEDADGY-FIRVPAGKKTSMPVQTCLMLGSKKAAQTVHNIIIVEEGATLDIITGCTTKKGVEEGLHLGISEMYIKKGGTLNFTMIHNWAEQIGVRPRTVVSVEEGGTYVSNYICLKPVRSVQTYPTVRLEGEGAVTRLNTIAIAHPGSELDLGSKAIFNAPGTRAELISRTITIGGRLIARGEMIGNAKGAKGHLECKGLVLTDKGSQLAIPILEANVDDIELTHEAAVGKIAKDQVEYLMARGLTEDEAVGMIIRGFLDVGIR----GIPEELKEEIENTIAQTALG----

General information: TITO was launched using: RESULT: Template: 4DN7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2299 31401 13.66 90.23 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 13.66 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_4DN7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DN7-query.scw PDB file : Tito_Scwrl_4DN7.pdb: