Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYCQFFSVLALGLSAASCAVTSGLQTYDIPSEGVYKTDLGTTVNVVKISQETLPAIQPAQIDYQRDYASLFKNQQTIYRLSPGDVLSIQLWAYPEIAPPVNNISNEQSVQAYGYPIDQTGYIQFPLVGRYKAAGKTLAQVNRELHSQLARFLKNPDVVVRVVSYEGQRFSVQGSVTKGGQF---YLSDQPVSIYTALGMAGGVTTTGDNTYIQLIRNGRT--------------------------------------------YNLNTIDLEKAGYSLHKLLVQPNDTIYVSTRENQKIYVMGESGKNQALPMRDQGMSLTDALGESLGINPLSGSASRIYVVRTNPNDRTTEIYHLNLMSIGDFGLANQFRLRSNDVVYVDATGL------TRWQRIVNQIIPFSNALYNIDRLGQ 3P42 Chain:D ((4-228)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMVTIYLPGEQQTLSVG-PVENVAQLVTQPQ-LRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKSVRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRP-VTITLLGAVSGAGQLPWLAG-RSVTDYLQDHPRL-A-GADKNNVMVITPEG---ETVVAPVALW------NKRHVEPPPGSQLWLGFSAHVLPEKYADLNDQIVSVLTQRVPE--------

General information: TITO was launched using: RESULT: Template: 3P42.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 599 303 0.51 1.76 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.56 3D Compatibility (PKB) : 0.51 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_3P42.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P42-query.scw PDB file : Tito_Scwrl_3P42.pdb: