Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MFVALSFDWVFFFGVNMDTIEAKKNLNALCNEIEKLQNLSRSLMTAKE---ML--DIDAKIKRHKEQVKNIRSNLHA-- 1KP6 Chain:A ((1-79)) NNAFCAGFGLSCKWECWCTAHGTGNELRYATAAGCG-----DHLSKSYYDARAGHCLFSDDLRNQFYSHCSSLNNNMSCRSLSK

General information: TITO was launched using: RESULT: Template: 1KP6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 266 -6164 -23.17 -91.99 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -23.17 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_1KP6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KP6-query.scw PDB file : Tito_Scwrl_1KP6.pdb: