Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYSEKVIDHYENPRNVGVLDKNSENVGTGMVGAPACGDVMRLQIQVNDN-GVIEEARFKTYGCGSAIASSSLVTEWLKGKTLDEAQAIKNIDIATELALPPVKVHCSVLAEDAIKAAIEDYRSKKSKA 1Q48 Chain:A ((1-127)) MAYSEKVIDHYENPRNVGSLDKKDSNVGTGMVGAPACGDVMQLQIKVD-DNGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLEEAGAIKNSQIAEELELPPVKVHCSILAEDAIKAAIADYKAKQGL-

General information: TITO was launched using: RESULT: Template: 1Q48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 502 18453 36.76 146.45 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.96 3D Compatibility (PKB) : 36.76 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_1Q48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q48-query.scw PDB file : Tito_Scwrl_1Q48.pdb: