Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSNMEVLIKTEDAIDAYNGAANLARSLGVSRAAISQWGEY--VPEVSARGLVI-----LQPSIPHIIKKPSAT 2HIN Chain:A ((1-66)) --------MKPEELVRHFGDVEKAAVGVGVTPGAVYQWLQAGEIPPLRQSDIEVRTAYKLKSDFTSQRMGKEGH

General information: TITO was launched using: RESULT: Template: 2HIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 221 -15736 -71.20 -266.71 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -71.20 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_2HIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HIN-query.scw PDB file : Tito_Scwrl_2HIN.pdb: