Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSARHSRLIILGSGPAGYSAAVYAARANLKPTLIAGLQL-----GGQLTTTTEVDNWPGDPEGLTGPVLMDRMQAHAERFGTELVYDHINEVDLNVRPFVLKGDMDE----YTCDALIIATGATAQYLGLESEQKFMGQGVSACATCDGF--FYKNQNVMVVGGGNTAVEEALYLSNIAEHVTLVHRRDSLRSEKILQDHLFAKEKESKISIVWNHEVEEVLGDNTGVTGVRLKSTKDDSKQEVQVQGLFIAIGHKPNTSMFEGQLNLRD-GYIQVQSGTSGNATATSVAGVFAAGDVADSIYRQAITSAGSGCMAALDAEKYLDNL 3ITJ Chain:B ((22-337)) ----HNKVTIIGSGPAAHTAAIYLARAEIKPILYEGMMANGIAAGGQLTTTTEIENFPGFPDGLTGSELMDRMREQSTKFGTEIITETVSKVDLSSKPFKLWTEFNEDAEPVTTDAIILATGASAKRMHLPGEETYWQKGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKVFMLVRKDHLRASTIMQKR---AEKNEKIEILYNTVALEAKGDGKLLNALRIKNTKKNEETDLPVSGLFYAIGHTPATKIVAGQVDTDEAGYIKTVPGSS----LTSVPGFFAAGDVQDSKYRQAITSAGSGCMAALDAEKYLTSL

General information: TITO was launched using: RESULT: Template: 3ITJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1886 68482 36.31 225.27 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : 36.31 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_3ITJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ITJ-query.scw PDB file : Tito_Scwrl_3ITJ.pdb: