Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPPRIRRSVMRQFTSLQVAILALGSLCFSTAYADSTLVPMSDAELSATRGQALMSMSYIAPNDSANLEKLRDSSSNVGFYKLGLEAELEINANIRKLQLGCGGVNGAGGCDIDFDNVSLSGVADTREGRVASDAKLTNPFLEFAIKNPNSASTREVAGIRLSAEAVEGLLTIGTENSATPNGINSLSGYMVVAPQVGEATVDAARITQTGSPACGVYPSPAGCGVNQAITGKARGKIALGAGFNLDFQTKSYDITLTPTQKAQLSLPQSVVSGQRMSSVNLLASAIVNGIDLSGTLAADVDILGGITLNGNLRGTINNLPVTVPLLENLGYIHKINLSGSPLSLSMQGQDIRWPGTVSTAMRGWWLELSNPIDIGRIDPTNSVVIKTDTIRDALTEVSKELTQNPLNCGFLAVNCIGGDFNVGTRDLSNARPALLELQNLQLANQSFAPNCYGSLKFC 5CZL Chain:A ((23-337)) LPRAWADT---AWESYKSRFM----------MADGRIVDTGNGSVSHTEGQGFAMLLAVAKNDRPAFDKLWQWTDKTLRNK------------------------DNGLFYWRYNPVAPDPIAD-KNDATDGDTLIAWALLRAQQQWGD-------------------------------------KSYGIASDAITASLLKSTVITFAGHQVM--LPGAKGFNRNDHVNLNPSYFI---FPAWQAFAARTHLTAWRKLQSDGKALLGKMAWGK--------AQLPSDWVALRAD--------GRMEPAKEWPPRMSYDAIRIPLYLSWA---------DPQS------------ALLTPWKSWFQSYP------RLQTPAWVNVNTNDV------APWFMTGGLLAVRDLTTGEAQDDPQLSAQD--DYYSASLKML-VWLAKNDR-----------

General information: TITO was launched using: RESULT: Template: 5CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1734 -88481 -51.03 -280.89 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -51.03 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_5CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CZL-query.scw PDB file : Tito_Scwrl_5CZL.pdb: