Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHMVTTKKAKVQFNWQDPFLIEQQLSPDERMIHDAAHAYCQERLQPRVLEQFRHETTDPSIFREMGELGLLGPTIPEQYGGAGLNYVSYGLVAREIEYVDSGYRSMASVQSSLVMVPIHEFGTEEQKQKYLPKLATGEYIGCFGLTEPDHGSDPGSMITRAKKVEGGYRLTGAKMWITNSPIADVFVVWAKEVSAEGNVGDICGFILEKGWEGLSAPAIHGKVGLRASITGEIVMDNVFVPEENAFPEIRGLKGPFTCLNSARYGIAWGAMGAAEFCWHTAHQYTMDRKQFGRPLAANQLIQKKLADMQTEIALGLQAALRFGRMKDEGIASVEGTSLIKRNNCGKALDIARMARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAQTGIAAFSN 3GNC Chain:C ((8-398)) -----------ATFHWDDPLLLDQQLADDERMVRDAAHAYAQGKLAPRVTEAFRHETTDAAIFREMGEIGLLGPTIPEQYGGPGLDYVSYGLIAREVERVDSGYRSMMSVQSSLVMVPIFEFGSDAQKEKYLPKLATGEWIGCFGLTEPN-----GSMVTRARKVPGGYSLSGSKMWITNSPIADVFVVWAK--LDEDGRDEIRGFILEKGCKGLSAPAIHGKVGLRASITGEIVLDEAFVPEENILPHVKGLRGPFTCLNSARYGIAWGALGAAESCWHIARQYVLDRKQFGRPLAANQLIQKKLADMQTEITLGLQGVLRLGRMKDEGTAAVEITSIMKRNSCGKALDIARLARDMLGGN---DEFGVARHLVNLEVVNTYEGTHDIHALILGRAQTGIQAF--

General information: TITO was launched using: RESULT: Template: 3GNC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2112 -166624 -78.89 -435.05 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.93 3D Compatibility (PKB) : -78.89 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3GNC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GNC-query.scw PDB file : Tito_Scwrl_3GNC.pdb: