Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRTPISFEFFPPKTDASAEKLRIVHQELQHLNPEFFSITYGAGGSTRERTLAAIEDFNGK-GTPVAPHLSCIGDDKTRIAELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAGANAGITQFFFNPDSYFYFIERLEKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHEVVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN 1B5T Chain:B ((5-272)) ------VSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDLPPGS-GKPEM-YASDLVTLLKEVA--DFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGV-

General information: TITO was launched using: RESULT: Template: 1B5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1314 -36316 -27.64 -136.01 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -27.64 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_1B5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B5T-query.scw PDB file : Tito_Scwrl_1B5T.pdb: