Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMGSKKQEEIMKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFVWHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHISEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNY-GGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYY---EVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEFIGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK 1GNX Chain:B ((15-478)) -----------ALTFPEGFLWGSATASYQIEGAAAEDGRTPSIWDTYARTPGR-VRNGDTGDVATDHYHRWREDVALMAELGLGAYRFSLAWPRIQPTGRG-PALQKGLDFYRRLADELLAKGIQPVATLYHWDLPQELENA-GGWPERATAERFAEYAAIAADALGDRVKTWTTLNEPWCSAFLGYGSGVHAPGRTDPVAALRAAHHLNLGHGLAVQALRDRLPADAQCSVTLNIHHVRPLTDSDADADAVRRIDALANRVFTGPMLQGAYPEDLVKDTAGLTDWSFVRDGDLRLAH-QKLDFLGVNYYSPTLVS------------HSPWPGADRVAFHQPP-GETTAMGWAVDPSGLYELLRRLSSDFPALPLVITENGAAFHDYAD--P-EGNVNDPERIAYVRDHLAAVHRAIKDGSDVRGYFLWSLLDNFEWAHGYSKRFGAVYVDYPT-GTRIPKASARWYAEVARTGVLPT------

General information: TITO was launched using: RESULT: Template: 1GNX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2684 -143199 -53.35 -323.25 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -53.35 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_1GNX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GNX-query.scw PDB file : Tito_Scwrl_1GNX.pdb: