Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPE-EFKSIVLSISKELELGVFPKVFVQKFS-PEVLGPPPSLVFFTD---EFETIYENMEEPG-E---ITDNNGILTFNFSDPEGNYFVIGKAESSD 2RK0 Chain:A ((2-129)) -----------------SLSGVSHVSLTVRDLDISCRWYTEILDWKELVRGRGDTTSFAHGVLPG--GLSIVLREHDGGGTDLFDETRPGLDHLSFSVESMTDLDVLEERLAKAGAAFTPTQELPFGWILAFRDADNIALEAMLGRE--

General information: TITO was launched using: RESULT: Template: 2RK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 432 -8056 -18.65 -67.70 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -18.65 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_2RK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RK0-query.scw PDB file : Tito_Scwrl_2RK0.pdb: