Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGFNLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEK-KVVLA-G-----GKKLDFDRLILATGSNQKFRNISA----ESTLIHPVKKIQNVTALQQKIM--NAKKIAIIGAGQAGIEIAEGLASRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVETEKRKENYDLVLLANHSRPDNQMWEE-QLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVTFRPTNEKMYVSLVNNAVRTARNVSKTISGSQ-TKDQGTYRPVGNQWFGYFLGSVGLTEEESIFYPQKIETGYVDYK--LTAIN-QSKVKLKFLFDSE-KKILGAQLQSKKEMFHLLDQLTIAVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED 3ICT Chain:A ((35-468)) GSRKIVVVGGVAGGASVAARLRRLSEEDEIIMVERGEYISFANCGLPYYIGGVITERQKLLVQTVERMSKRFNLDIRVLSEVVKINKEEKTITIKNVTTNETYNEAYDVLILSPGAKPIVPSIPGIEEAKA--LFTLRNVPDTDRIKAYIDEKKPRHATVIGGGFIGVEMVENLRERGIEVTLVEMANQVMPP-IDYEMAAYVHEHMKNHDVELVFEDGVDALEENGAVVRLKSGSVIQTDMLILAIGVQPESSLAKGAGLALGVRGTIKVNEKFQTSDPHIYAIGDAIEVKDFVTETETMIPLAWPANRQGRMLADIIHGHTDSLYKGTLGTSVAKVFDLTVATTGLNEKILKRLNIPYEVVHVQANSHAGYYPNATPVLIKLIFNKDSGKIYGAQTLGRDGVDKRMDVIATAIKANLTVLDLPDLELSYAPPYSS---------------

General information: TITO was launched using: RESULT: Template: 3ICT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2380 5507 2.31 13.27 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 2.31 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_3ICT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ICT-query.scw PDB file : Tito_Scwrl_3ICT.pdb: