Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIYIASRIAERLKQLEDIDIPVGELDITLYRDD-KKENPEEPELHSSDIPVSLEGKEVILIDDVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEILVEMQELDGQDRIMILKEED 4P86 Chain:B ((5-182)) ----KAVILDEQAIRRALTRIAHEMIERNKGMNNCILVGIKTRGIYLAKRLAERIEQIEGNPVTVGEIDITLYRDDL--TSNDEPLVKGADIPVDITDQKVILVDDVLYTGRTVRAGMDALVDVGRPSSIQLAVLVDRGHRELPIRADYIGKNIPTSKSEKVMVQLDEVDQNDLVAIYENE-

General information: TITO was launched using: RESULT: Template: 4P86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 889 -67135 -75.52 -385.83 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : -75.52 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.628

(partial model without unconserved sides chains): PDB file : Tito_4P86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P86-query.scw PDB file : Tito_Scwrl_4P86.pdb: