Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEGQWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNGEDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKENPKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGIE-AREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQEYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIEELIQLGMNRYHQRQSFFEKNE 2ZDG Chain:D ((1-319)) MRVLLIAGGVSPEHEVSLLSAEGVLRHIPF---PTDLAVIAQDGRW----LLGEK--ALTALEAKAAPEGEHP-------FPPPLSWERYDVVFPLLHGRFGEDGTVQGFLELLGKPYVGAGVAASALCMDKDLSKRVLAQAGVPVVPWVAVRKGEPPVVPFDPPFFV----------KPANTGSSVGISRVERFQDLEAALALAFRYDEKAVVEKALSPVRELEVGVLGNVFGEASPVGEVRYEAP------------AELLIPAPL---TQETVQELALKAYKVLGVRGMARVDFFLA-EGELYLNELNTIPGFTPTSMYPRLFEAGGVAYPELLRRLVELALT-------------

General information: TITO was launched using: RESULT: Template: 2ZDG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1747 -76748 -43.93 -253.29 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : -43.93 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_2ZDG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZDG-query.scw PDB file : Tito_Scwrl_2ZDG.pdb: